Publications

Nature Methods (accepted)

Voltage imaging of neurons distributed across entire brains of larval zebrafish

Zeguan Wang*, Jie Zhang*, Panagiotis Symvoulidis, Wei Guo, Davy Deng, Adam Amsterdam, Lige Zhang, Takato Honda, Steven Roche, Matthew A Wilson, Edward S Boyden (* Co-first authors)

Summary: A high-speed remote-scanning light-sheet microscope (rsLSM) enabled voltage imaging of ~25% of neurons distributed throughout the entire larval zebrafish brain at 200 Hz.

Neuron (2024)

A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events

Yukun A Hao*, Sungmoo Lee*, Richard H Roth, Silvia Natale, Laura Gomez, Jiannis Taxidis, Philipp S O’Neill, Vincent Villette, Jonathan Bradley, Zeguan Wang, Dongyun Jiang, Guofeng Zhang, Mengjun Sheng, Di Lu, Edward Boyden, Igor Delvendahl, Peyman Golshani, Marius Wernig, Daniel E Feldman, Na Ji, Jun Ding, Thomas C Südhof, Thomas R Clandinin, Michael Z Lin (* Co-first authors)

Summary: The ASAP5 voltage indicator was developed with enhanced activation kinetics and improved responsivity near resting membrane potentials, enabling better detection of both spiking and subthreshold activity.

Nature Communications (2024)

Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy

Jie Zhang#, Jonathan Newman, Zeguan Wang, Yong Qian, Pedro Feliciano-Ramos, Wei Guo, Takato Honda, Zhe Sage Chen, Changyang Linghu, Ralph Etienne-Cummings, Eric Fossum, Edward Boyden, Matthew Wilson (# Corresponding author)

Summary: Camera sensors with pixel-wise programmable exposure phase and timing enhanced the temporal resolution and signal-to-noise ratio of voltage imaging.

Cell (2023)

Temporally multiplexed imaging of dynamic signaling networks in living cells

Yong Qian, Orhan T Celiker, Zeguan Wang, Burcu Guner-Ataman, Edward S Boyden

Summary: Genetically encoded fluorescent proteins with distinct switching kinetics allowed standard microscopes to simultaneously image and separate multiple cellular signals within a single living cell using a single color channel.

IEEE International Conference on Computational Photography (ICCP) (2021)

Real-time light field 3D microscopy via sparsity-driven learned deconvolution

Josue Page Vizcaino, Zeguan Wang, Panagiotis Symvoulidis, Paolo Favaro, Burcu Guner-Ataman, Edward S Boyden, Tobias Lasser

Summary: Deep learning networks accelerated the 3D reconstruction of light-field images by more than a thousand-fold.

Optica (2020)

Sparse decomposition light-field microscopy for high speed imaging of neuronal activity

Young-Gyu Yoon*, Zeguan Wang*, Nikita Pak, Demian Park, Peilun Dai, Jeong Seuk Kang, Ho-Jun Suk, Panagiotis Symvoulidis, Burcu Guner-Ataman, Kai Wang, Edward S Boyden (*co-first authors)

Summary: Sparse decomposition algorithm enhanced the resolution and signal-to-noise ratio of light-field microscopy by exploiting the spatiotemporal sparsity of neuronal activity, enabling near single-cell–resolution whole-brain imaging at 50 Hz.

Biomedical Optics Express (2018)

Optical volumetric projection for fast 3D imaging through circularly symmetric pupil engineering

Bo Cai, Xiaomin Zhai, Zeguan Wang, Yan Shen, Ronald Xu, Zachary J Smith, Quan Wen, Kaiqin Chu 

Summary: A specially designed optical mask enabled microscopes to simultaneously focus across thick volumes, allowing fast 3D imaging of biological samples.

eLife (2017)

Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

Lin Cong*, Zeguan Wang*, Yuming Chai*, Wei Hang*, Chunfeng Shang, Wenbin Yang, Lu Bai, Jiulin Du, Kai Wang#, Quan Wen# (*co-first authors, #co-corresponding authors) 

Summary: Novel light-field microscope and tracking system enabled rapid whole-brain calcium imaging in freely behaving zebrafish, and while the animal chased and captured prey.